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Microscope: Zeiss LSM 710 Confocal

Microscope: Zeiss LSM 710 Confocal

LOCATION

George R Brown, W 125B (New location)

PRICING

Rice User Fee: $25/hr prime-time (8am-8pm) and $10/hr off-peak time (8pm-8am and weekends)

Non-Profit Fee: $39.25 prime-time (8am-8pm) and $15.65/hr off-peak

For Profit Fee: Please ask Dr. A. Budi Utama. ext 713-348-8232 or email budiutama@rice.edu

Rice Training Fee: $50/hr

Non-Profit Training Fee: $78.50/hr

CONTACTS

Training Contact:

To request training, contact Dr. A. Budi Utama at ext. 713 348 8232 or email budiutama@rice.edu or Harshavardhan Deshmukh - hd16@rice.edu
Training takes 3 sessions. The first session is 2 hours of training, and the second session is about 2-3 hours of supervised practice. The third session takes about 1 hour to check out. It is recommended that the three training sessions be finished in two weeks and when the users are ready with their own sample for experiment.

DESCRIPTION

The Inverted Confocal microscope system is based on Axio Observer Z1 MOT.

More specifications include:

  • 6 lasers lines (405, 458, 488, 514, 543 and 633 nm).
  • PMT detector for fluorescence and transmitted/DIC light
  • Transmitted light and X-Cite fluorescence Illuminator.
  • Two galvanometric scanning confocal heads (for DIC/Phase Contrast and Fluorescence imaging).
  • Motorized stage 130x85 for x,y,z movement.
  • Master pinhole.
  • Optics: EC Plan-Neofluar 10x/0.30 WD=5.2, Plan-Apochromat 20x/0.8 WD=0.55; Plan-Apochromat 40x/0.95 Corr WD=0.25; EC Plan-Neofluar 40x/1.3 DIC WD=0.21; Plan-Apochromat 63x/1.40 Oil DIC
  • AxioCam MRm Rev.3 camera for regular transmitted/fluorescence widefield imaging.
  • Definite Focus system for longer term live cells imaging.
  • A complete on-stage (small) set of environmental control system (CO2, temperature and humidity control).
  • Anti-vibration table.
  • Zeiss ZEN 2010 software.
  • PC and 30’ monitor.