BioLector I High-throughput, Real-time Monitoring, Automated Sampling Micro-Fermentation System
The BioLector I is a bench top micro-fermentation system to perform high-throughput fermentations together with online monitoring of the several fermentation parameters, including biomass, pH, DO and fluorescent molecules.
Applications of the device include cell line and strain screening, media screening and optimization, fermentation parameter optimization, anaerobic and microaerophilic fermentations, protein building kinetics, high-throughput protein expression, enzyme and cell activity tests, functional genomics, proteomic studies, and inhibition and toxicity tests.
 
System basic parameters:

• Working volume of 200– 800– 2400 µL [DEPENDING ON MICRO-TITER PLATE AND CONDITIONS]
• 48 or 96 parallel micro-reactions
• Humidity control (> 75 % relative Humidity)
• Temperature control (5 °C below room temperature) with active cooling
• Orbital shaker 400 – 1500 rpm at 3 mm (diameter)
• Anaerobic chamber with [N2 or CO2] available

Specific available parameters for this device include optodes measuring:

• Biomass: Ex 620 nm, Em 620 nm
• pH: Ex 470 nm, Em 525 nm [READING REQUIRES BioLector® PLATE WITH CORRELATING OPTODE SENSOR]
• Dissolved Oxygen (pO2): Ex 520 nm, Em 600 nm [READING REQUIRES BioLector® PLATE WITH CORRELATING OPTODE SENSOR]
• Fluorescent molecules sensors available:
  • GFP: Ex 488 nm, Em 520 nm
  • Riboflavin: Ex 436 nm, Em 540 nm
  • NADH/NADPH: Ex 365 nm, Em 450 nm
  • dsRed I, RFP I, tdTomato: Ex 550 nm, Em 580 nm
  • nile red: Ex 589 nm Em 671 nm