This microscope is designed to combine a 1-photon laser point-scanning confocal with an alternate 2-photon module for deeper penetration into thick samples.

Excitation Laser Lines:

Four regular electronic solid-state laser lines (405, 488, 560, and 640 nm) and an addition of NIR 730 nm laser lines for the confocal system.

The Insight X3 Dual-OL 2-photon laser has 100-fs pulses across a 680-1300nm tuning range for the 2-photon system (see below for details).

Optics:

• UPLXAPO 4X, 0.16 NA, 13mm WD
• UPLXAPO 10X, 0.40 NA, 3.1mm WD
• UPLXAPO 20X, 0.80 NA, 0.6mm WD
• UPLSAPO 30x Silicone IR, 1.05 NA, 0.8mm WD, 370-1300 nm excitation wavelength.
• UPLXAPO 40X, 0.95 NA, 0.18mm WD
• UPLXAPO 60x Oil, 1.42 NA, 0.15mm WD

Detectors:

• 2 channels broadband Non-Descan Detector for 2-photon epifluorescence light path.
• One regular transmitted light detector, T-PMT.
• Total of 4 SilVIR detectors included (cooled SiPM, 2 blue-shifted type, 2 red-shifted type)

The game-changing SilVIR detector combines two advanced technologies—a silicon photomultiplier (SiPM) and Olympus' patented fast signal-processing design. This silicon photomultiplier (SilVIR/SiPM) type detector eliminates many of the issues and restrictions of conventional PMTs. The Olympus patented signal processing circuit (SilVIR) also incorporates technology that maximizes the sensor’s capabilities, expanding its applicability to high-pixel numbers and high-speed imaging. A very high S/N ratio and very high sensitivity with almost no background (extremely low noise), combined with a very large dynamic range, allow users to adjust laser power to get an outstanding image.  High dynamic range photon counting (16-bit) and no degradation of sensitivity.

Others:

• LED-based wide-field transmitted light and fluorescence Illuminators.
• Hybrid Galvanometric and Resonant scanner head provides software-selectable galvanometer and resonance scanners for high definition or high-speed imaging in a single system.
• Motorized X/Y-stage.
• Motorized Z drive with 10nm resolution and 3mm/sec maximum movement speed.
• Olympus TruFocus Z-Drift compensation system utilizes a minimally phototoxic beam to accurately maintain the focus position.
• Condenser Focus Stop – The unique Olympus IX3 Condenser Refocusing Stopper allows customers to easily reproduce Koehler illumination settings.
• Motorized DIC objective prism can be programmed to automatically disengage during fluorescence acquisition to maximize fluorescence resolution.
• Ultra-fast acousto-optic tunable filters (AOTF) laser control facilitates high-speed laser switching with pixel-precise beam blanking and attenuation of the laser lines.
• FLUOVIEW acquisition & analysis software package enables a multitude of acquisition and analysis features, including 3D image acquisition and display, ratio imaging analysis and display, live 16-channel spectral unmixing, time-lapse imaging, FRAP, and FRET acquisitions and fluorescence intensity measurements.
• Rice University Networked HP Z8G5 Windows 11 confocal PC with Dell 43” UHD 4K monitor.
• Tokai-Hit Warming Box with Nose Piece heater to ensure the correct temperature can be maintained for all objectives. A live-cell environmental-stage top incubator is available to use.
• Sample holder for slide/dish and well plates.
• Anti-vibration table.

 Spectra Physic InSight X3 Dual-OL Pulsed IR 2-Photon Laser

The Insight X3 Dual-OL produces short is optimized for use with the Olympus microscopes. It delivers 100-fs pulses across a wide 680-1300nm tuning range and a second fixed-wavelength output to facilitate the most demanding multiphoton imaging experiments.

The InSight X3 Dual-OL tunable output features DeepSee™ dispersion compensation, delivering the shortest pulses to the sample. The DeepSee can be tuned to maximize image brightness, allowing deeper tissue imaging with lower risk of optical damage.

Power SPECIFICATIONS:

> 1.0 W @ 700 nm

> 2.0 W @ 900 nm

> 1.8 W @ 1000 nm

> 1.6 W @ 1100 nm

> 1.4 W @ 1200 nm

> 1.0 W @ 1300 nm

Dual Output:

> 3.5 W at 1045 nm (Fixed)