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LSM 980

Microscope: Zeiss LSM980 Airyscan-2 Confocal

LOCATION

O’CONNOR B16

PRICING

Rice User Fee: $45/hr

Non-Profit Fee: $70.50/hr

For Profit Fee: $225/hr

Rice Training Fee: $90/hr

Non-Profit Training Fee: $141/hr

For Profit Training Fee: $450/hr

CONTACTS

Training Contact:

A. Budi Utama – Optical Imaging Facility Manager, budiutama@rice.edu or x-8232

Faculty in Charge:

James McNew -Professor of BioSciences, mcnew@rice.edu

DESCRIPTION

This microscope is designed to combine laser point-scanning confocal with a super-resolution Airyscan-2 module, which allows faster and improved resolution for fixed and live-cell imaging. The Airyscan-2 module works for Single Laser Point Scanning (SR mode), Multiplex Four Laser Point Scanning (4Y), Multiplex Eight Laser Point Scanning (8Y) and Confocal Eight Laser Point Scanning (Co-8Y). Hence, it is possible to increase the scanning speed eight times faster than the regular single-point scanning speed under the Galvano scanner within the same imaging area.

 

The Airyscan-2 super-resolution doubles up to 120 nm from 240 nm resolution in confocal. In SR mode, the resolution can be further improved to 90 nm using the ZEN3 module Airyscan Joint Deconvolution. This works in 2D and 3D images.

 

Our Zeiss LSM980 Inverted Confocal and Airyscan-2 super-resolution microscope system is based on the AxioObserver-7 Inverted Microscope.

 

 

Excitation Laser Lines:

Four regular electronic solid-state laser lines (405, 488, 560, and 640 nm) and an addition of NIR 730 nm laser lines.

 

Optics:

  • Zeiss EC Plan-Neofluar 10x/0.30; ∞/-, WD=5.20 mm (Cat# 420340-9901).
  • Zeiss Plan-Apochromat 20x/0.8; ∞/0.17, WD=0.55 mm, (Cat# 420650-9903).
  • Zeiss Plan-Neofluoar 40x/1.30 ∞/0.17, Oil DIC, WD=0.21 mm, (Cat# 420462-9900).   
  • Zeiss Plan-Apochromat 63x/1.4 DIC, ∞/0.17, Oil DIC, WD=0.28 mm, (Cat# 420782-9900-7991). The objective of choice for Airyscan super-resolution use.   
  • Zeiss Plan-Apochromat 100x/1.4 Oil DIC, WD=0.19 mm, (Cat# 420792-9900).

 

Detectors:

  • One 4-Channel GaAsP Spectral Array Detector (upgradeable to 34-channel detection).
  • Two multi-alkali PMT detectors.
  • One 32-channel concentric Airyscan GaAsP detector.
  • One NIR GaAsP detector.
  • One NIR GaAS detector.
  • One Transmitted Light Detector T-PMT.

 

Others:

  • LED Transmitted light and X-Cite fluorescence Illuminators.
  • AI Sample Finder for easy identification of sample holders and automatic tissue detection.
  • Axiocam 305 Monochrome Camera.
  • Two galvanometric scanning confocal heads.
  • Motorized X/Y-stage and Piezo-Z-stage.
  • Master pinhole.
  • Definite Focus module.
  • Live cell environmental stage top incubator.
  • Sample holder for slide/dish and well plates.
  • Anti-vibration table.
  • Zeiss ZEN Blue software with modules for Molecular Quantification Module for FRAP, FLIP, FRET, Photoactivation, Airyscan and Airyscan 2 Multiplex plus, Sample Navigator, Tiles/Positions, Stitching, Experiment Designer, Direct Processing, Airyscan Joint Deconvolution.
  • Rice University Networked Win10 PC and 24’ monitor.

 

 

Resources:

- Useful video links for Zeiss LSM800 Airyscan:  

https://bitesizebio.com/product_article/zeiss-airyscan-a-brave-new-microscopy-world-with-sharper-confocal-resolution/

-Reading material from Zeiss LLC: https://www.zeiss.com/microscopy/us/l/campaigns/multiplex-imaging-solutions.html?utm_source=google&utm_medium=cpc&utm_campaign=C-00008031&gad_source=1&gclid=EAIaIQobChMI87gpJPGhwMVCqiDBx1WCSZVEAAYASAAEgKM3PD_BwE